| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Reduction of adhesion properties of Ruditapes philippinarum hemocytes exposed to Vibrio tapetis
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| Autores: |
Choquet, Gwenaelle
Soudant, P
Lambert, C
Nicolas, Jean-louis
Paillard, C
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| Data: |
2003-12
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| Ano: |
2003
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| Palavras-chave: |
Cytotoxicity
Flow cytometry
Vibrio tapetis
Ruditapes philippinarum
Bivalve hemocytes
Pathogenicity
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| Resumo: |
Vibrio tapetis is the causative agent of brown ring disease (BRD), which affects a species of clam, Ruditapes philippinarum. After incubation with V. tapetis, hemocytes lose filopods and become rounded, indicating cytotoxic activity of the bacterium. To rapidly quantify this cytotoxicity, a flow-cytometry test was developed based on the capacity of V tapetis to inhibit adhesion of clam hemocytes to plastic. Several bacteria:hemocyte ratios, the cytotoxicity of other Vibrio spp. pathogenic to bivalves, and that of various V. tapetis isolates were tested. Inhibition of adherence is detectable with as few as 5 bacteria per hemocyte. The greater cytotoxic activity of V tapetis compared to that of V splendidus and V pectenicida suggests a specific pathogenicity of V. tapetis to R. philippinarum hemocytes. Although all V. tapetis isolates inhibited adhesion, significant variations in cytotoxicity among isolates was demonstrated.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00000/10761/7590.pdf
DOI:10.3354/dao057109
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| Editor: |
Inter-research
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| Relação: |
http://archimer.ifremer.fr/doc/00000/10761/
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| Formato: |
application/pdf
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| Fonte: |
Diseases Of Aquatic Organisms (0177-5103) (Inter-research), 2003-12 , Vol. 57 , N. 1-2 , P. 109-116
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